Genetics of Laboratory Animals and Experimental Models

Coordinación

Module coordinators:
Belen Pintado
Centro Nacional de Biotcnología, Madrid, Spain.

Sergio Berdún
Parc Cientific de Barcelona and Veterinary School of Barcelona, Spain

Teaching staff: 

Joaquim Alves da Silva 
Champalimaud Research, Lisbon, Portugal

Antonio Armario
Universitat Autònoma de Barcelona, Spain

Salvador Bartolome
Laboratori de Luminescència i Espectroscòpia de Biomolècules,,UAB, Spain

Fernando Benavides
MD Anderson Cancer Center, Austin, Texas

Sergio Berdun
Parc Cientific de Barcelona and Veterinary School of Barcelona, Spain

Cory Brayton
Johns Hopkins Medicine, Baltimore, USA

James Bussell
Biomedical Services Department. University of Oxford, United Kingdom

Agustí Casadesús
Envigo CRS SA Spain.

Mariano Domingo Álvarez
CReSA-IRTA, Spain

Verónica Domínguez Plaza
CNB-CSIC, Madrid Spain

Carme Fabregat Roig
Center for Regenerative Medicine, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain

Fernando Angel Fernández Alvarez
Institut de Ciències del Mar, Barcelona, Spain

Graziano Fiorito
Stazione Zoologica Anton Dohrn, Napoli, Italy

Miriam Illa
​Foetal Medicine Research Center, Hospital Sant Joan de Deu, Barcelona, Spain

Jean Jaubert
Institute Pasteur, Paris, France

Eduard Martin
HIPRA, Amer, Spain

Lluís Montoliu
Centro Nacional de Biotecnología, CNB-CSIC, Madrid, Spain

Ángel Naranjo
Centro Nacional de Biotecnología, CNB-CSIC, Madrid, Spain

Teresa Paramio
Universitat Autonoma de Barcelona, Spain

Joan Perez
HIPRA, Amer, Spain

Belén Pintado
Centro Nacional de Biotecnología, CNB-CSIC, Madrid, Spain

Anna Pujol
CBATEG, Universitat Autònoma de Barcelona. Spain

Juan Ramos
Universitat Autonoma de Barcelona, Spain.

Montserrat Rigol
IDIBAPS, Barcelona, Spain

Mercè Roca
HIPRA, Amer, Spain

Jesús Ruberte
CBATEG, Universitat Autònoma de Barcelona, Spain

Manuel Sanchez Martín
Transgenesis service, Cancer Research Centre (CRC), Salamanca Spain

Violeta Solis
GSK, Madrid, Spain.

Marta Terré
Centre Torre Marimón (IRTA), Spain

Javier Terriente
ZeClinics, Barcelona, Spain

Graham Tobin
Envigo RMS, UK

Toni Ventura
Center for Regenerative Medicine, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain

Roger Villanueva
Institut de Ciencies del Mar, Barcelona, Spain

David P Wolfer
Neuroscience Center, Zurich, Switzerland

English for webinars, lectures and course materials. For practical sessions groups for both English and Spanish speakers.

Duration of the module: 
15 ECTS, (88 attendance hours)
Cost: 

€3,600 (may be made in two instalments of €1,800)

Course dates: 

15 February -15 May 2023

Attendance dates: March 6 to 16, 2023

Descripcion

This module provides the practical and theoretical knowledge and skills regarding genetics and experimental models required by designated veterinarians and other onsite personnel responsible for the welfare and care of laboratory animals in the conduct of experimental procedures.

The module consists of two parts. The experimental models component provides extensive knowledge of different experimental animal models in different research areas. The genetic component explores models of genetically modified animals, their production, maintenance and the implications of their use. 

The content of this module also complies with the training requirements set out in European Directive EU63/2010, developed by the Expert Working Group, supported by European Commission, in respect of the following modules: 3.2) Basic and appropriate biology (theory); 7) Minimally invasive procedures without anaesthesia (theory), 8) Minimally invasive techniques without anaesthesia (skills).

For details, see: https://ec.europa.eu/environment/chemicals/lab_animals/pdf/Endorsed_E-T.pdf

Syllabus: 

Topic 1.Genetics, genetic control and genetically modified rodents.  

1.1.Basic Concepts of the genetic of mammals. 
1.2.Genetically defined lines of mice. 
1.3.Genetic modification: transgenesis, targeted mutagenesis and genome editing. Tools and applications 
1.4.Genetically defined lines of rats and other rodents used in animal experiments. Spontaneous mutations. 
1.5.Genetic markers used in laboratory rodents 
1.6.Genetic control and genetic contamination. Practical cases 
1.7.Standard Nomenclature of mice and rats including GA animals 
1.8.Rodent embryo manipulation. Collection, culture and transfer. Comparing mice and rats 
1.9. Genetic altered models in fish
1.10.Management of colonies. Breeding strategies to keep consanguinity and avoid consanguinity. Breeding schemes in genetically altered animals 
1.11.Influence of genetic background on phenotype, examples in neurobiology 
1.12.Epigenetics, concept and impact on genotype and phenotype
1.13.Rederivatio 
1.14.The 3Rs in GA models. How to use international resources 
1.15.Production and Management of GA colonies for Phenotyping Platforms 
1.16.Rodent basic phenotyping
1.17.Phenotypes by image 
1.18.Phenotypes by behaviour 
1.19.Reproductive biotechnology. Cryopreservation. FIV;
1.20.From founder to line: Establishing a Genetically Altered (GA) line
1.21.Establishing a new genetically altered line according to Directive 2012/63. Practical issues
1.22.Rat husbandry differences in relation to mice
1.23.Practical sessions: 

1.23.1. Genotyping procedures 
1.23.2.  Abdodminal and scrotal vasectomy
1.23.3.  Obtaining sperm and FIV
1.23.4.  Cryopreservation 2 cell and morulae
1.23.5.  Collection and Transfer of mouse1-cell embryos 
1.23.6.  Phenotyping behaviour

Topic 2. Selection of animal model according to biological characteristics 

2.1. Nutrition and its influence on research
2.2.. Biology, management and care guidelines in cephalopods
2.3. Management, administration and taking samples in the main species of experimental animals: rodents, rabbits, pigs, ruminants, birds, fish, amphibians, cephalopods and demonstation on dogs and non-human primates

Topic 3 Selection of experimental model according to type of research. 

3.1.Introduction to animal models
3.2. Experimental models in neurodegenerative and rare diseases
3.3. Experimental models in cardiovascular diseases
3.4. Experimental models in oncology.
3.5. Experimnental models in diabetes and metabolic diseases
3.6. Experimental models in fetal medicine
3.7. Experimental models in Reproduction
3.8. Experimental models in immunology
3.9. Experimental models in infectious diseases.

Registration or more information:

Tel:(+34) 935 811 848
e-mail: pg.animals.laboratori@uab.cat