The course consists of on-line theoretical lessons and practical exercises followed by hands on practical lessons for the species selected.
The on-line theoretical lessons are expected to be completed in a maximum period of 3 months. Practical sessions will be organized on demand and there will not be a delay of more than 3 months between completion of the on-line part of the course and the hands-on practical sessions.
The course includes an evaluation consisting of: 1) individual assessment of practical skills, 2) MCQ exam of basic knowledge and 3) solving 4 case studies related to the individual student's research project.
Both the skill assessment and the basic knowledge exam will be done in person at the UAB.
MODULE 1: CONDUCTING ANIMAL EXPERIMENTS
Lesson 1. Introduction to LAS. Ethical Principles and Legislation
Lesson 2. Animal models.
2.1. Animal model definition, choice of the animal model. Translation from animal studies to humans
2.2. Genetically modified animals.
Lesson 3. Factors influencing the experiment (I): Quality Standardization
3.1. Genetic standardization
3.2. Microbiology standardization
Lesson 4: Factors influencing the experiment (II): Environmental factors
4.1. Nutrition and feeding.
4.2. Animal facilities and procedures/ legal requirements for centre registration
4.3. Health monitoring and prevention of disease
Lesson 5. Factors influencing the experiment (III): Animal welfare.
5.1. Animal Welfare. Transport of animals
Lesson 6. Recognition of Pain, suffering and distress and its alleviation. Refinement
6.1. Anaesthesia and analgesia.
6.2. Recognition of Pain
6.3. Animal identification, basic principles of surgery, post surgical care
Lesson 7. Health and Safety
MODULE 2: DESIGNING PROCEDURES AND PROJECTS
Lesson 1: Experimental design
2.1 Factors influencing experimental design. Examples of experimental design
2.2. Estimation of sample size. Practical approach to statistics.
Lesson 2. Design of procedures and projects
2.1 Literature search. Systematic reviews.
2.2. Alternatives to animal experimentation
2.3. Harmonization and standardization
2.4. Preparation of protocols for ethical evaluation. Severity evaluation. Non technical summaries
2.5 Reporting animal studies
MODULE 3: SPECIES BIOLOGY AND EXPERIMENTAL PROCEDURES
Mice, rats, rabbits, pigs, ruminants, birds, fish, amphibians, cephalopods