Genetics of Laboratory Animals and Experimental Models

Coordinación

Module coordinators:
Belen Pintado
Centro Nacional de Biotcnología, Madrid, Spain.

Sergio Berdún
Parc Cientific de Barcelona and Veterinary School of Barcelona, Spain

Teaching staff: 

María Abad 
Fundació Institut d’ Oncologia Vall de Hebron, VHIO, Barcelona, Spain

Antonio Armario
Universitat Autònoma de Barcelona, Spain

Salvador Bartolome
Laboratori de Luminescència i Espectroscòpia de Biomolècules,,UAB, Spain

Fernando Benavides
MD Anderson Cancer Center, Austin, Texas

Sergio Berdun
Parc Cientific de Barcelona and Veterinary School of Barcelona, Spain

James Bussell
Sanger Institute, Reino Unido

Raquel Cabezón
IDIBAPS, Barcelona, Spain

Agustí Casadesús
Envigo CRS SA Spain.

Evelyne Célérier
Panlab, S.L. Barcelona, Spain

Mariano Domingo Álvarez
CReSA-IRTA, Spain

Elisenda Eixarch
Hospital Sant Joan de Deu, Barcelona, Spain

Carme Fabregat Roig
Center for Regenerative Medicine, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain

Graziano Fiorito
Stazione Zoologica Anton Dohrn, Napoli, Italy

Sylvie Franckhauser
Centre de Biotecnologia Animal Teràpia Gènica (CBATEG). UAB. Barcelona, Spain

Tomás García Robles
​Barcelona Biomedical Research Park (PRBB), Spain

Jean Jaubert
Institute Pasteur, Paris, France

Ricard March
HIPRA, Amer, Spain

Eduard Martin
HIPRA, Amer, Spain

Lluís Montoliu
Centro Nacional de Biotecnología, CNB-CSIC, Madrid, Spain

Ángel Naranjo
Centro Nacional de Biotecnología, CNB-CSIC, Madrid, Spain

Teresa Paramio
Universitat Autonoma de Barcelona, Spain

Belén Pintado
Centro Nacional de Biotecnología, CNB-CSIC, Madrid, Spain

Anna Pujol
CBATEG, Universitat Autònoma de Barcelona. Spain

Juan Ramos
Barcelona Biomedicine Research Parc, Spain.

Montserrat Rigol
IDIBAPS, Barcelona, Spain

Jesús Ruberte
CBATEG, Universitat Autònoma de Barcelona, Spain

Jorge Sztein
Envigo RMS, Spain

Marta Terré
Centre Torre Marimón (IRTA), Spain

Javier Terriente
ZeClinics, Barcelona, Spain

Graham Tobin
Envigo RMS, UK

Toni Ventura
Center for Regenerative Medicine, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain

Roger Villanueva
Institut de Ciencies del Mar, Barcelona, Spain

David P Wolfer
Neuroscience Center, Zurich, Switzerland

 

English for lectures and course materials. For practical sessions groups for both English and Spanish speakers

Duration of the module: 
15 ECTS, (88 attendance hours)
Cost: 

3,600 (may be made in two instalments of €1,800)

Course dates: 

15 February -15 May 2022

Attendance dates: to be determined

Descripcion

This module provides the practical and theoretical knowledge and skills regarding genetics and experimental models required by designated veterinarians and other onsite personnel responsible for the welfare and care of laboratory animals in the conduct of experimental procedures.

The module consists of two parts. The experimental models component provides extensive knowledge of different experimental animal models in different research areas. The genetic component explores models of genetically modified animals, their production, maintenance and the implications of their use. 

The content of this module also complies with the training requirements set out in European Directive EU63/2010, developed by the Expert Working Group, supported by European Commission, in respect of the following modules: 3.2) Basic and appropriate biology (theory); 7) Minimally invasive procedures without anaesthesia (theory), 8) Minimally invasive techniques without anaesthesia (skills).

For details, see: http://ec.europa.eu/environment/chemicals/lab_animals/pdf/Endorsed_E-T.pdf

Syllabus: 

Topic 1.Genetics, genetic control and genetically modified rodents.  

1.1.Basic Concepts of the genetic of mammals. 
1.2.Genetically defined lines of mice. 
1.3.Genetic modification: transgenesis, targeted mutagenesis and genome editing. Tools and applications 
1.4.Genetically defined lines of rats and other rodents used in animal experiments 
1.5.Genetic markers used in laboratory rodents 
1.6.Genetic control and genetic contamination. Practical cases 
1.7.Standard Nomenclature of mice and rats including GA animals 
1.8.Rodent embryo manipulation. Collection, culture and transfer. Comparing mice and rats 
1.9.Management of colonies. Breeding strategies to keep consanguinity and avoid consanguinity. Breeding schemes in genetically altered animal 
1.10.Management of colonies of transgenic animals: Legal issues Creation and statistical counting in practice 
1.11.Influence of genetic background on phenotype, examples in neurobiology 
1.12.Rederivatio 
1.13.The 3Rs in GA models. How to use international resources 
1.14.Production and Management of GA colonies for Phenotyping Platforms 
1.15.Establishing a new genetically altered line according to Directive 2012/63. Basic phenotypation. Practical issues 
1.16.Phenotypes by image 
1.17.Phenotypes by behaviour 
1.18.Reproductive biotechnology. Cryopreservation. FIV 
1.19.Genetically altered models in fish 
1.20.Practical sessions: 

1.20.1. Genotyping procedures 
1.20.2.  Abdodminal and scrotal vasectomy
1.20.3.  Obtaining sperm and FIV
1.20.4.  Cryopreservation 2 cell and morulae
1.20.5.  Collection and Transfer of mouse1-cell embryos 
1.20.6.  Phenotyping behaviour

Topic 2. Selection of animal model according to biological characteristics 

2.1 Nutrition and its influence on research
2.2. Biology, management and care guidelines in cephalopods
2.3. Management, administration and taking samples in the main species of experimental animals: rodents, rabbits, dogs, pigs, ruminants, nonhuman primates, birds, fish, amphibians and cephalopods.

Topic 3 Selection of experimental model according to type of research. 

3.1.Introduction to animal models
3.2 Experimental models in neurodegenerative and rare diseases
3.3 Experimental models in cardiovascular diseases 3.4. Experimental models in oncology.
3.5 Experimnental models in diabetes and metabolic diseases
3.6 Experimental models in fetal medicine
3.7 Experimental models in Reproduction
3.8 Experimental models in immunology
3.9. Experimental models in infectious diseases.

Registration or more information:

Tel:(+34) 935 811 848
e-mail: pg.animals.laboratori@uab.cat